What experimental approach(es) would you devise to test the specific hypothesis: that specialized ribosomes exist for differential translation of testis-specific mRNAs in Drosophila?
Note that all molecular, biochemical, genetic, and cellular reagents (e.g., DNA clones, antibodies, fly strains) available from Kearse et al. (2011, 2013) are available to you for your experimental approach. If other reagents are needed for the experiments that you devise, you must include a strategy for making those necessary reagents as well as the experimental approach for using the reagents.
Question 2. What experimental approach(es) would you devise to test the specific hypothesis that testis-specific post-translational modifications including SUMOylation and phosphorylation are required to localize eRpL22 to the nucleoplasm of meiotic spermatocytes in Drosophila?